Brain PS 840032 L-α-phosphatidylserine (Brain, Porcine) (sodium salt)

Brain phosphatidylserine (PS) is a porcine tissue-derived anionic phospholipid commonly found in neuronal membranes. Chemically identified as L-α-phosphatidylserine (Brain, Porcine) sodium salt, this lipid reflects the composition naturally present in mammalian brain cells.

Within healthy cells, phosphatidylserine resides primarily on the inner leaflet of the plasma membrane and is maintained there through active lipid transport processes. When PS relocates to the outer membrane surface, it functions as a biological signal involved in several tightly regulated cellular events.

The fatty acid profile (C18:0 at 42%, C18:1 at 30%, C22:6 DHA at 11%) of this product mirrors authentic porcine brain tissue, providing a physiologically relevant lipid composition for membrane and apoptosis-related studies.

Applications

• Apoptosis and efferocytosis modeling: Used to prepare PS-rich liposomes that mimic apoptotic membranes for studies examining phosphatidylserine-binding proteins such as TIM-4, BAI1, and Annexin V, as well as macrophage recognition and clearance pathways.
• Coagulation biology assays: Incorporated into synthetic phospholipid vesicles used to assemble prothrombinase and tenase complexes, supporting investigations of clotting factor activity and platelet -associated procoagulant responses.
• Neuroscience membrane reconstitution: The brain-derived fatty acid composition provides a biologically relevant lipid environment for experiments exploring PS-dependent protein interactions linked to synaptic signaling, tau aggression, and α-synuclein membrane binding.

Formulation & handling

Brain PS dissolves readily in chloroform:methanol:water (65:25:4) at concentrations exceeding 5mg/mL; it does not dissolve in ethanol or DMS. For aqueous preparations, evaporate solvent under nitrogen, hydrate the dry lipid film with aqueous buffer, then vortex or sonicate to disperse. Extrude through polycarbonate membranes for uniform vesicle populations.

Researchers should consider the product’s sodium salt form when calculating surface charge in mixed lipid systems. This lipid absorbs moisture and degrades with repeated temperature cycling, so handle under dry, inert conditions and divide into aliquots when possible. Store at −20 °C in a dark environment. Brain PS is stable for approximately 3 months at the recommended storage conditions.

Avanti Research™ supplies Brain PS at >99% purity, manufactured with strict quality standards to support sensitive biological assays.

References

Fadok, V. A., de Cathelineau, A., Daleke, D. L., Henson, P. M., & Bratton, D. L. (2001). Loss of phospholipid asymmetry and surface exposure of phosphatidylserine is required for phagocytosis of apoptotic cells by macrophages and fibroblasts. The Journal of biological chemistry, 276(2), 1071–1077. https://doi.org/10.1074/jbc.M003649200

Frasch, S. C., & Bratton, D. L. (2012). Emerging roles for lysophosphatidylserine in resolution of inflammation. Progress in lipid research, 51(3), 199–207. https://doi.org/10.1016/j.plipres.2012.03.001

Kim, H. Y., Huang, B. X., & Spector, A. A. (2014). Phosphatidylserine in the brain: metabolism and function. Progress in lipid research, 56, 1–18. https://doi.org/10.1016/j.plipres.2014.06.002

Segawa, K., & Nagata, S. (2015). An Apoptotic 'Eat Me' Signal: Phosphatidylserine Exposure. Trends in cell biology, 25(11), 639–650. https://doi.org/10.1016/j.tcb.2015.08.003

Zwaal, R. F., Comfurius, P., & Bevers, E. M. (1998). Lipid-protein interactions in blood coagulation. Biochimica et biophysica acta, 1376(3), 433–453. https://doi.org/10.1016/s0304-4157(98)00018-5