LNP Lipid Blend 2 A83213 (Previously 300213) LNP Lipid Blend 2

This pre-mixed lipid blend is composed of components suitable for producing nucleic acid-based lipid nanoparticles.  Works best with DNA/plasmid cargo, and can be used for other nucleic acids as well.

Instructions for using the LNP Lipid Blend 2:

1. Add 1-mL of ethanol to the vial to achieve 25 mg/mL. Mix well to achieve full dissolution.*

2. Dilute nucleic acid in aqueous buffer.**

3. Use solvent injection to create LNPs. Different parameters and cargo will result in different particle size results. 

4. Dialyze the final formulation against buffer of choice to remove residual ethanol.

*Note: low heat, sonication, or vortexing can be used as needed

**Note: common buffers include citrate, Tris, or PBS