MSI SPLASH™ 330841 MSI SPLASH™ LIPIDOMIX for Mass Spec Imaging

MSI SPLASH™ LIPIDOMIX for Mass Spec Imaging

MSI SPLASH™ LIPIDOMIX is a precisely defined internal standard mixture developed for Mass Spectrometry Imaging (MSI) of lipids in biological tissues, particularly neurological tissues.

As researchers continue to uncover the connections between endogenous lipid dysregulation and neurological or neurodegenerative diseases, spatial lipid profiling has become essential for exploring the biochemical organization of the brain and other tissues at their tissue surfaces and within their extracellular matrices.

Designed in collaboration with experienced MSI researchers and optimized for compatibility with both MALDI and DESI workflows, this MSI internal standard mixture enables both quantitation and spatial localization of diverse lipid classes across heterogeneous tissue matrices within a single MSI experiment. By offering a consistent and reproducible reference signal, it enhances data comparability, supports cross-sample analysis, and improves confidence in the biological interpretation of spatial lipidomics data.

What MSI SPLASH™ enables

Mass spectrometry imaging requires accurate normalization to compare lipid abundance across different regions, samples, or experimental conditions. MSI SPLASH™ LIPIDOMIX provides a multiclass, isotope-labeled internal standard mixture that is evenly applied to tissue sections to form a uniform quantitative reference layer. This lipid imaging approach supports:

• Reliable normalization of ion intensities across imaging regions
• Improved reproducibility between instruments, operators, and studies
• Simultaneous quantitative and spatial lipid analysis in tissues
• Confident interpretation of lipid distribution patterns in situ

Designed in collaboration with leading MSI research groups, MSI SPLASH™ LIPIDOMIX ensures reproducible, cross-sample lipid quantitation and supports both MALDI and DESI imaging workflows, thereby advancing neurobiological and spatial lipidomics studies.

Applications for MSI SPLASH™ LIPIDOMIX

Researchers use MSI SPLASH™ LIPIDOMIX in a range of spatial lipidomics and neuroscience studies and have been able to take advantage of its ability to deliver accurate, reproducible, and high-resolution results for:

• Spatial lipidomics of brain and neural tissues
• Studies on Parkinson's, Alzheimer's, ALS, and other neurodegenerative conditions
• Mapping lipid distributions across anatomical or histological regions
• Multimodal workflows integrating MSI with histopathology or LC-MS lipidomics
• Development and validation of standardized MSI processing and quantitation protocols

These applications rely on the internal standards within MSI SPLASH™ LIPIDOMIX to stabilize ion signals and ensure consistent comparative data.

Component composition

Containing thirteen isotope-labeled lipid standards that represent major lipid classes typically present in mammalian tissues, this MSI SPLASH™ LIPIDOMIX mixture from Avanti Research™ supports comprehensive, omics-scale analysis in MSI-based lipidomics experiments.

Supporting research for validation

MSI SPLASH™ LIPIDOMIX was developed through collaboration with Michiel Vandenbosch, Shadrack Mutuku, Shane Ellis, and Kim Ekroos, leading experts in mass spectrometry imaging and quantitative lipidomics.

The efficacy of MSI SPLASH™ is thoroughly explored and validated in Vandenbosch, M. et al. “Toward Omics-Scale Quantitative Mass Spectrometry Imaging of Lipids in Brain Tissue Using a Multiclass Internal Standard Mixture.” Analytical Chemistry (2023) 95(51): 18719–18730. https://doi.org/10.1021acs.analchem.3c02724.

This peer-reviewed study demonstrates that MSI SPLASH™ enables robust quantitative and spatial lipid analysis across neurological tissues, setting a new benchmark for reproducibility in MSI workflows.