Cyanine 5 NHS 810320 1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E)-5-((E)-1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium chloride

Cyanine 5 NHS is an amine-reactive fluorescent probe that combines standard Cyanine 5 with an ester from the N-hydroxysuccinimide (NHS) group. Although it is sometimes called Cy5 dye, as an amine-reactive NHS ester, it is more accurately described as a fluorescent probe for biomolecule conjugation.

This ester derivative probe enables rapid and efficient conjugation to primary amines such as lysine residues on proteins, peptides, and other biomolecules; it's the superior choice for imaging and flow cytometry applications that demand clarity and signal integrity.

Researchers can expect exceptional brightness, impressive photostability, and minimal background interference from Cyanine 5 NHS due to its far-red excitation around 650 nm and strong emission near 670 nm.

Here at Avanti Research™, we specialize in high-quality research reagents designed to meet the rigorous demands of advanced life science applications. With over 50 years of experience and a proven track record across thousands of laboratories, we continue to set the standard for scientific precision with uncompromising adherence to high-quality practices.

Cyanine 5 NHS enables straightforward labeling in amine-free buffers such as PBS or MES, with optimal performance observed when conjugating proteins at 10 mg/mL or higher concentrations. Boasting extinction coefficients up to 250,000 M⁻¹cm⁻¹ and quantum yields near 0.2, it delivers performance on par with Cy5 probes trusted by core imaging facilities and flow cytometry labs worldwide.

Users should be aware that cyanine fluorescent probes can undergo blue-conversion artifacts during multicolor imaging; following proper photoprotection protocols helps preserve fluorescence fidelity throughout extended experiments.