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18:1 PA 840875 1,2-dioleoyl-sn-glycero-3-phosphate (sodium salt)
18:1 PA is 1,2-dioleoyl-sn-glycero-3-phosphate (sodium salt), a highly pure phosphatidic acid (PA) species commonly referred to as DOPA (PA(18:1/18:1)). As a central intermediate in glycerophospholipid metabolism and a bioactive membrane lipid with a defined fatty acid composition, 18:1 PA (DOPA) is widely used as a model PA species in lipid research. Its structural uniformity supports reproducible biochemical and biophysical studies involving membrane dynamics, curvature modulation, vesicle trafficking, and effector protein recruitment in signaling pathways.
Product overview
Phosphatidic acid (PA), the simplest glycerophospholipid, functions as both a central precursor in glycerophospholipid biosynthesis and a bioactive signaling lipid that regulates membrane-associated proteins. Structurally, PA is an anionic lipid composed of a glycerol backbone esterified to two fatty acids and a phosphate group.
Supplied in sodium salt forms, 18:1 PA (DOPA) contains two oleoyl (18:1) acyl chains, providing a defined unsaturated PA species. Its dioleoyl composition supports fluid bilayer formation and dynamic membrane behavior, making it well-suited for model membrane systems. With a transition temperature of −8 °C, 18:1 PA is frequently incorporated into liposomes and other reconstituted systems to investigate membrane curvature, fusion and trafficking events, and PA-dependent effector recruitment under controlled experimental conditions.
Applications
- Model membrane systems & liposome formulation: Phosphate sodium salt (DOPA) is incorporated into LUVs, GUVs, and supported lipid bilayers to study membrane organization, lipid packing, and dynamic membrane behavior in controlled systems.
- Membrane curvature & fusion assays: This lipid compound's anionic headgroup and unsaturated dioleoyl chains make it useful for investigating curvature-driven remodeling, budding, and membrane fusion processes.
- Lipid–protein interaction studies: 18:1 PA is used to evaluate PA-binding proteins and domains in recruitment, binding, and reconstitution assays requiring a defined phosphatidic acid species.
- Enzymology & PA metabolism workflows: Substrate or defined reference lipid in studies involving phospholipases and PA metabolic pathways.
- Analytical standards & method development: Structurally uniform PA species for chromatographic and LC–MS method development and validation.
Formulation, handling & storage
Available from Avanti Research™ in sodium salt forms, as a hygroscopic powder or dissolved in chloroform.
To ensure optimal stability of this product for up to one year:
- Allow material to equilibrate to room temperature before opening to avoid condensation.
- Aliquot upon first opening to reduce repeated handling.
- Prepare solutions using clean, dry glassware and handle quickly at room temperature.
- Recap promptly after each use.
- Store at −20 °C (protected from ambient temperatures, light, and moisture).
- Solubility Note: 18:1 PA is insoluble in ethanol and DMSO and is soluble in chloroform:methanol:water (65:25:4) at 5 mg/mL for stock preparation prior to membrane incorporation.
For bulk quantities or technical performance data, contact Avanti Research.
Sources
- https://pubmed.ncbi.nlm.nih.gov/37103336/
- https://pmc.ncbi.nlm.nih.gov/articles/PMC9687397/
- https://www.mdpi.com/2218-273X/8/2/20
