DSPE-PEG2k-tri mannose 880161 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000]-N-tri mannose, ammonium salt
DSPE-PEG2K-tri mannose from Avanti Research™ is a PEGylated phospholipid designed to introduce mannose receptor recognition and targeted delivery applications in advanced lipid nanoparticle (LNP) and liposome-based drug delivery systems.
Anchored by DSPE—a phospholipid with two long hydrophobic stearoyl chains—the DSPE-PEG2k-tri mannose conjugate embeds into liposomes, lipid nanoparticles, or similar bilayer structures, while the polyethylene glycol (PEG 2000) spacer provides sufficient steric extension to present the tri-mannose ligand cluster for receptor recognition at the cell surface.
This multivalent mannose design enhances cellular uptake through C-type lectin receptors such as mannose receptors (MR) on immune cells, including those expressed on dendritic cells, B cells, macrophages, and other antigen-presenting cells. It supports receptor-mediated targeted gene delivery, facilitating intracellular trafficking of nucleic acid or antigen payloads for downstream protein expression and immune response activation.
Beyond immune cell targeting, mannosylated PEG lipids have shown enhanced uptake and specific accumulation in hepatic immune populations—particularly Kupffer cells and liver sinusoidal endothelial cells—offering utility for liver-directed targeting strategies, liver diseases, or liver cancer models following intravenous injection in in vivo evaluation studies.
Applications
DSPE-PEG2K-tri mannose is a PEG lipid commonly used to surface-functionalize lipid carriers in studies involving:
- Receptor-mediated cellular uptake by dendritic cells or macrophages
- Antigen or immune-modulating cargo delivery
- Vaccine research and immunology model development
- Investigation of cell–ligand recognition and internalization pathways
This PEG lipid plays a critical role in supporting research where targeted delivery and selective association with antigen-presenting cells are desired for mechanistic or delivery-focused studies.
Formulation and handling
We offer DSPE-PEG2k-tri mannose for research-use only (not for therapeutic or clinical applications) in an ammonium salt powder form, compatible with aqueous handling and solvent-assisted formulation workflows.
Incorporate DSPE-PEG2K-tri mannose into lipid formulations at experimentally optimized ratios to tune ligand surface density. Store at −20°C, protected from light and moisture, and allow the vial to warm to room temperature before opening to prevent condensation.
Packaging & specifications
Avanti Research™ supplies DSPE-PEG2k-tri mannose in defined, quality-controlled quantities (10 mg units or in bulk by request) with batch-specific certificates of analysis to support reproducibility and verify purity and composition (average molecular weight 3393 Da due to PEG polydispersity).
Supporting citations
Mannose-decorated LNPs and liposomes are widely used for targeting strategies that enhance the specific accumulation of these particles in target cells, including immune cells, those in the bone marrow, and liver-associated immune populations. Studies demonstrate statistically significant enhancement of cellular uptake and antigen delivery compared with viral vectors or positive control formulations (Int J Mol Sci. 2024; “Mannose Ligands for Mannose Receptor Targeting” https://pmc.ncbi.nlm.nih.gov/articles/PMC10855088/).
