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Hydro Soy PC 840058 L-α-phosphatidylcholine, hydrogenated (Soy)
Hydro Soy PC (HSPC) is a hydrogenated phosphatidylcholine derived from natural soy lecithin. During hydrogenation, the unsaturated fatty acids present in native lecithin are converted primarily into saturated acyl chains. The result is a highly stable phospholipid mixture composed mainly of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC, ~88.6%), along with a smaller amount of 1-palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine (PSPC, ~11.4%).
Because the fatty acyl chains are fully saturated, HSPC exhibits a relatively high gel-to-liquid crystalline phase transition temperature (Tm) of approximately 53 °C. Bilayers composed of HSPC are tightly packed and relatively rigid, exhibiting improved oxidative stability and reduced permeability compared with bilayers formed from unsaturated phospholipids. These characteristics make Hydro Soy PC particularly useful in liposomal systems where structural stability and low membrane permeability are essential.
Hydrogenated soy phosphatidylcholine is widely used in pharmaceutical liposome formulations and serves as a principal bilayer component in several well-known liposomal therapeutics, including the FDA-approved Doxil® and AmBisome®. In these formulations, the lipid bilayer contributes to drug retention and supports extended circulation when combined with other formulation components such as cholesterol and PEGylated lipids.
Applications
- PEGylated liposome formulations: Combined with cholesterol and mPEG200-DSPE at a 56:38:5 molar ratio, HSPC can form rigid, ordered bilayers near physiological temperature when incorporated into PEGylated liposome formulations.
- Passive tumor targeting: Low-permeability bilayers help retain encapsulated drugs and support tumor accumulation in formulations designed to exploit the enhanced permeability and retention (EPR) effect, a mechanism underlying Doxil®-type oncology formulations.
- Antifungal therapy: HSPC is the primary bilayer component of AmBisome®, a liposomal formulation of amphotericin B used for systemic fungal infections.
- Oral and pH-responsive delivery: Used in controlled-release matrices prepared by hot-elt extrusion and pH-sensitive systems designed to release payload in the tumor microenvironment.
- Biophysical research: HSPC liposomes are a frequently used model system for studying surface interactions, boundary lubrication, and bilayer structure; common characterization methods include cryo-TEM, SAXS/WAXS, and AF4-MALS.
Formulation & handling
HSPC dissolves in ethanol and in chloroform:methanol:water mixtures (e.g., 65:25:4) at concentrations around 5 mg/mL. It has limited solubility in DMSO.
For liposome preparation, combine it with the desired lipid components in an organic solvent. Remove the solvent by rotary evaporation at a temperature above its Tm (~53°C) to create a thin lipid film. Hydrate the film using an aqueous buffer heated to 55–65 °C, allowing the lipid bilayers to form efficiently.
This material is not hygroscopic and exhibits minimal light sensitivity. Store at –20 °C; when kept under these conditions, the product remains stable for up to one year.
Avanti Research™ produces Hydro Soy PC (HSPC) with >99% purity and tightly controlled fatty-acid composition to ensure reliable performance across batches.
