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Mini Extruder Pro Assembly Instructions

Before assembling the extruder for the first time, thoroughly clean all parts (except for the Polycarbonate Membranes and the Filter Supports) with a mild detergent solution, followed by rinsing with hot tap water and deionized or distilled water. Allow all parts to dry before assembling apparatus.

The new, improved Mini Extruder Pro design contains a stainless steel outer casing and retainer nut, two internal membrane supports, and two o-rings that fit into the channel of the internal membrane supports. The Mini Extruder Pro should be disassembled and cleaned after each extrusion batch and rinsed with methanol or acetone to dry. 

How To Assemble Mini Extruder Pro

Refer to the diagram to identify the parts in these instructions.

Mini Extruder Pro Assembly Instructions

1. Place the 2 Internal Membrane Supports on a flat surface with the O-rings facing up.

Mini Extruder Pro Place the 2 Internal Membrane Supports on a flat surface with the O-rings facing up 

2. Pre-wet a filter support with DI water, or buffer, and center inside the o-ring. The filter support should adhere to the teflon orifice inside the O-ring inner diameter. 

Mini Extruder Pro Pre-wet a filter support with DI water, or buffer, and center inside the o-ring. The filter support should adhere to the teflon orifice inside the O-ring inner diameter.  

3. Insert the Internal Membrane Support, with the Filter Support, into the Extruder Outer Casing with the O-ring facing up.

Insert the internal membrane support, with the filter support, into the extruder outer casing with the O-ring facing up.

4. Place one thin, shiny polycarbonate membrane over the filter support and O-ring. Do not install the blue separator paper. 

Place one thin, shiny polycarbonate membrane over the filter support and O-ring. Do not install the blue separator paper.

IMPORTANT NOTE: Polycarbonate Membranes and Filter Supports are intended to be used for a single liposome preparation and should not be reused.  

5. Pre-wet a second filter support with DI water, or buffer, and place over orifice of remaining internal membrane support. 

Pre-wet a second filter support with DI water, or buffer, and place over orifice of remaining internal membrane support.

6. Carefully place the second internal membrane support into the casing (O-ring facing down) being careful not to twist the membrane support when it comes in contact with the membrane. It is best to lift the internal membrane support up inside the stainless steel outer casing to prevent movement of the polycarbonate membrane due to creation of vacuum. 

Carefully place the second internal membrane support into the casing (O-ring facing down) being careful not to twist the membrane support when it comes in contact with the membrane. 

7. Place the retainer nut on the threaded end of the extruder outer casing and tighten.

Place the retainer nut on the threaded end of the extruder outer casing and tighten.  

IMPORTANT NOTE:  Autoclaving the Mini Extruder Pro is not recommended as slight distortion may occur (-0! 002mm). The use of ethylene oxide or gamma irradiation is suggested as an alternative. The syringes should be sterilized with ethanol.

Warranty

Avanti Polar Lipids, LLC guarantees the apparatus free of defects of workmanship for a period of thirty (30) days. If you experience any problems due to workmanship during this time, please contact us to arrange an immediate replacement or exchange of the defective component.