TopFluor™ Derivatives
TopFluor™ (also referred to as BODIPY) is an extremely versatile fluorophore that can be structurally modified to fulfil a variety of roles. TopFluor™ is an extremely versatile fluorophore due to its chemical makeup and its high tolerance for substitutions which results in a compound that can be derivatized to suit a researcher’s needs.
TopFluor™-labeled lipids are great aids in studying the distribution of lipids, lipid diffusion rates, and lipid dynamics. TopFluor™ has a tunable fluorescence emission range from 500 - 700nm, high fluorescent quantum yields regardless of the solution, and a high level of photostability. TopFluor™ has also been used to study accessibility in the lipid bilayer, cellular localization, and membrane dynamics via fluorescence recovery. Studies have indicated that this fluorescent label alters the biochemical and biophysical properties of lipids less than some other fluorescent labels, such as NBD, while also maintaining a low toxicity, low polarity, and a high degree of biocompatibility.
